Q01045: Identification of species origin of milk and milk products using ELISA and PCR
Tuesday 12 August 2003
This research project aims to develop a method than can be used to determine the species origin of milk products used in milk and cheese.
Background
The ability to determine the species origin of milk products is important in protecting consumer interests. Two independent methods are currently used qualitatively in commercial assays for the determination of the species origin of milk used in milk and cheese, and are based on immunoassay and molecular techniques.
Research Approach
The project aims to develop ELISA (enzyme linked immunosorbent assay) and PCR (polymerase chain reaction) methods that will identify and quantify cows milk as an adulterant of sheep, goat and buffalo milks, in both raw/pasteurised milk and cheese produced from these milks. The end-point will be a series of assays suitable to take to validation trial and commercialisation. A close collaboration with a company (Tepnel Biokits) will facilitate commercialisation and will ensure that all reagents are suitable for conversion to the market.