B16006: Cockle cell extracts - an in vitro cytotoxicity study of effects on lactate dehydrogenase leakage, ATP levels and apoptosis induction in Balb/c 3T3 and HepG2 cells
Thursday 14 October 2004
This research project aims to examine the potential cytotoxicity of cockle extract that gives rise to the atypical response in the diarrhetic shellfish poisoning (DSP) mouse bioassay (MBA).
Background
Since June 2001, atypical responses have been observed during the diarrhetic shellfish poisoning (DSP) mouse bioassay (MBA) used within the UK statutory biotoxin monitoring programme. These have occurred primarily from cockles from England, Wales and Northern Ireland, but also from small numbers of mussels from England and Wales. This project will examine the potential cytotoxicity of cockle extract that give rise to the atypical response in the DSP MBA.
Research Approach
This research project aims to examine, using conventional cell-based assays, any potential cytotoxicity of cockle extracts that gives rise to the atypical response in the DSP assay. The study will determine cytotoxicity by investigating whether the cockle extracts adversely affect the capacity of cells to function normally, degrade cell membranes and/or initiate programmed cell death.
Results and findings
Cockle extracts (positive or negative for the atypical diarrhetic shellfish poisoning (DSP) response) did not induce cytotoxicity when tested using the endpoints LDH release, Caspase activity, and ATP levels in either Balb/c 3T3 or HepG2 cells.
Dissemination information
The final report is available from the FSA Library and Information centre.
To obtain a copy, please contact the Enquiry Desk, Dr Elsie Widdowson Library and Information Services, Food Standards Agency (tel: 020 7276 8181/8182 or email: library&info@foodstandards.gsi.gov.uk)
Contact: For any enquiries concerning this research project, please contact the relevant Programme contact or email science@foodstandards.gsi.gov.uk