G03031: Factors influencing transgene survival and transfer in the rumen
Friday 18 April 2008
Background
This study addresses the possibility that the transfer of genes may occur from transgenic food/feed to gut microbes. Transgenic plant material, particularly genetically modified (GM) maize, is already used widely in animal feedstuffs in many parts of the world. Many more experimental GM plant lines are being explored by plant biotechnologists that might offer a variety of agronomic, health or nutritional benefits in the future, and that might therefore be utilised as feed components. The potential for gene transfer therefore needs to be considered with respect to the microbiota inhabiting the gastrointestinal tract of farm animals.
Research Approach
In vitro modelling of the rumen, together with real-time polymerase chain reaction (PCR) detection of specific gene sequences will be used to examine the release and survival of DNA during the degradation of GM plant material. In addition, model transgene constructs carrying multiple selectable marker genes will be used to study factors that influence acquisition of external DNA by the gut microflora, including antibiotic treatment and sequence homology (similarity) with resident genes. The marker genes will allow selection of transformed gut bacteria, and their recovery from metagenomic libraries. Any gene transfer detected will be rigorously confirmed by analysis of flanking sequences.