N05032: Expression polymorphism following alpha-tocopherol supplementation in healthy subjects
Thursday 3 April 2003
This research project aims to investigate the effect of alpha-tocopherol supplementation on individuals.
Background
The scientific evidence from in vitro studies strongly supports the hypothesis that alpha-tocopherol can modulate gene expression, both via its antioxidant role and also as intracellular signalling molecule. In addition, several clinically beneficial outcomes are reported following a-tocopherol supplementation. Using a proteomic approach, the effect of a-tocopherol supplementation on the expression of the plasma proteome will be determined. Samples from healthy volunteers who received placebo or a-tocopherol (200mg or 400mg for 28 days) will be screened for expression polymorphisms. Plasma & nasal lavage cells will be examined with a combination of 2-D gel electrophoresis and mass spectrometry to determine changes in protein expression. Data obtained will provide useful new information regarding the basal expression and responsiveness to a-tocopherol, of a number of proteins which may be important in the atherosclerotic process.
Research Approach
Using a proteomic approach, the effect of alpha-tocopherol supplementation on the expression of the plasma proteome will be determined.
- The proteins in plasma and nasal lavage cell samples from healthy volunteers who received placebo or a-tocopherol will be separated using 2-D gel electrophoresis.
- Separated protein samples will be examined and quantified by mass spectrometry to determine changes in protein expression.
- Expression polymorphisms will be identified.
- Data obtained will be interpreted with reference to the individual's antioxidant status, which was determined under a previous contract (N05027).