Infectivity and abnormal prion protein in tissues from sheep exposed to atypical scrapie (ongoing)

Last updated:
22 July 2007
This project will establish distribution of abnormal PrP and infectivity in sheep tissues, such as blood, muscle, central nervous system(CNS) and milk.
Study duration: July 2007 to June 2013
Project code: FS231050 (M03058)
Contractor: ENVT

Background

Recently identified atypical scrapie cases in sheep represent an emerging TSE of which little is known. Incidence in EU countries seems to vary between 3 and 11 per 10,000 tested animals. However, because there is probably a lack of sensitivity in screening tests towards this particular TSE, this value is likely to be an underestimation. There is no consistent information concerning the abnormal PrP or infectivity distribution in peripheral tissues of atypical cases. This absence of data, combined with the involvement of ARR/ARR (genotype most resistant to BSE) animals that were believed to be resistant to TSE, represent a major concern with regards to the efficiency of the current food protection policy against TSE.

Research Approach

This project will establish distribution of abnormal PrP using various tools including Immunohistochemistry, and infectivity (using transgenic mice model) in sheep tissues such as blood, muscle, central nervous system(CNS) and milk. The research will give consideration to the fact that atypical cases could be either a spontaneous affection from CNS origin or an acquired infectious disease resulting from an oral contamination.

Results

Additional Info

Dissemination

Published Papers

  1. Andréoletti, O., Orge, L., Benestad, S.L., Beringue, V., Litaise, C., Simon, S., Le Dur, A., Laude, H., Simmons, H., Lugan, S., Corbière, F., Costes, P., Morel, N., Schelcher, F. & Lacroux, C. (2011) Atypical/Nor98 scrapie infectivity in sheep peripheral tissues. Published online PLoS Pathogens 7: e1001285